Figure 5
From: Adaptable, illumination patterning light sheet microscopy
Illumination patterning in live zebrafish larvae (42% TAG amplitude). Scale bars = 100 μm. (a,b) Tg(kdrl:GFP) zebrafish (5 dpf), (c,d): Tg(h2afva:h2afva-EGFP) (5 dpf). (a) Fully illuminated plane through head and trunk section. (b) Patterning along x by phase-delayed illumination pulsing. (c) Fully illuminated plane through head and trunk section. Cyan box corresponds to ROI for (d). (d) Patterning along x by phase-delayed illumination pulsing. White line shows y-averaged illumination intensity. Red line shows position of beam focus for calibration performed in fluorescent dye solution.
