Figure 1

Electrophoretic patterns of purified complexes I, III and IV from Euglena gracilis. Isolated mitochondria were solubilized with n-dodecyl-β-D-maltoside followed by a two-step chromatographic purification (Complete gels used are shown in Suppl Information Fig. S5). (M) Total mitochondrial proteins from Euglena gracilis. (I) purified NADH:ubiquinone oxidoreductase (complex I) fraction, (III) enriched cytochrome bc1 complex (complex III) fraction, remaining complex IV could be observed, (IV) enriched cytochrome c oxidase (complex IV) fraction. Estimated molecular masses and identities of Coomassie brilliant blue-stained protein complexes are indicated (see Suppl. Information Fig. S1 for more details).