Figure 4 | Scientific Reports

Figure 4

From: The atypical subunit composition of respiratory complexes I and IV is associated with original extra structural domains in Euglena gracilis

Figure 4

3D resolution of the polypeptides that constitute the Euglena mitochondrial cytochrome c oxidase. Monomeric purified complex IV was resolved by BN-PAGE. The band of interest was excised and resolved in a glycine-SDS-PAGE (12% acrylamide). The 2D gel was then subjected to 3D tricine-SDS-PAGE (14% acrylamide) and stained with Coomassie brilliant blue. The identified subunits are labeled. NI, not identified; UP, unnamed protein with no homolog in other species. Details about subunit identification are given in Table 3.

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