Figure 1
From: Reciprocal cross-species induction of outer membrane vesicle biogenesis via secreted factors
P. aeruginosa ΔpqsA was grown to early stationary phase in defined medium. Cells were then removed and exposed to exogenous PQS for 2 hours in fresh defined medium. OMVs were harvested by filtration (0.45 μm) followed by ultracentrifugation, then analyzed by NTA and lipid analysis. (A) Average concentration and size distribution of OMVs in ΔpqsA cultures +/− exogenously added PQS. (B) Fold increase in OMV production for ΔpqsA in the presence of exogenous PQS (relative to 0 µM PQS). In both (A) and (B), wild type PA14 OMV production under “−PQS” experimental conditions is included for reference. Exogenous [PQS] in “+PQS” = 6.7 ± 1.4 µM. (C) Analysis of PQS induced vesicles produced in rich (LB) or defined medium (MOPS) by lipid assay. Statistical significance was analyzed by one-tailed Student t-Test. (****p < 0.0001, **p < 0.01, *p < 0.05, n = 4).
