Figure 5
From: Reciprocal cross-species induction of outer membrane vesicle biogenesis via secreted factors
PQS was extracted from OMVs using a 1:1 ethyl acetate ratio. The organic layer was dried under Nitrogen and the resulting sample was resuspended in 50% methanol. PQS was analyzed using reverse-phase HPLC as in Fig. 4. Chromatograms show compounds extracted from OMVs of (A) E. coli (+/−PQS), (B) K. pneumoniae (+/−PQS), and (C) P. mirabilis (+/−PQS). PQS retention time: 28.9 minutes. Exogenous [PQS] in “+PQS” = 6.7 ± 1.4 µM.
