Figure 3
Mass spectrometry analysis on GzmB-cleaved DEJ substrates. (a) Extracellular domain schematics for α6 integrin. GzmB mediated cleavage sites identified proteomically by ATOMs (red arrows) fall within the ligand-binding domains in the β-propeller region. GzmB, granzyme B; TM, transmembrane helix. (b) Extracellular domain schematics for β4 integrin. GzmB mediated cleavage sites identified proteomically by ATOMS (red arrows) falls within ligand-binding domains in the specificity-determining loop. (c) Extracellular domain schematics for collagen VII. GzmB mediated cleavage sites identified proteomically by ATOMS (red arrows) falls in the von Willebrand factor A and fibronectin type III-2 domains, which mediate collagen VII attachment to other dermal-epidermal junction components, such as laminins and collagen IV. TM, transmembrane helix; PSI, plexin-semaphorin-integrin; VWFA, von Willebrand factor A; CRR, cysteine-rich region; FNIII, fibronectin-like III domain; NC, non collagenous region; CMP, cartilage matrix protein; VWFA1/2, von Willebrand factor A 1/2; Pi, protein inhibitor.
