Figure 5 | Scientific Reports

Figure 5

From: Downregulation of WNT11 is associated with bladder tissue fibrosis in patients with interstitial cystitis/bladder pain syndrome without Hunner lesion

Figure 5

Nuclear localization of phosphorylated SMAD2 protein induced by downregulation of WNT family genes. (a–e) Immunofluorescent staining for nuclear localization of phosphorylated SMAD2 protein (green) in HBlEpC cells at 4 days after infection with lentivirus containing shWNT11 (a), shWNT2B (b), shWNT5A (c), shWNT10A (d), and shHB-EGF (e) constructs (magnification x200, scale bar = 100 μm). The higher magnification images (magnification x400, scale bar = 50 μm) are inserted in lower left corner in each image. Nuclei were stained with DAPI (blue). A scramble shRNA construct was used as the control.

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