Figure 6 | Scientific Reports

Figure 6

From: Downregulation of WNT11 is associated with bladder tissue fibrosis in patients with interstitial cystitis/bladder pain syndrome without Hunner lesion

Figure 6

Induction of vimentin intermediate filament protein by silencing of WNT family genes. (a–e) Immunofluorescent staining of vimentin (green), an intermediate filament protein induced by the EMT process in HBlEpC cells at 4 days after infection with lentivirus containing shWNT11 (a), shWNT2B (b), shWNT5A (c), shWNT10A (d), and shHB-EGF (e) constructs (magnification x200, scale bar = 100 μm). The higher magnification images (magnification x400, scale bar = 50 μm) are inserted in lower left corner in each image. Nuclei were stained with DAPI (blue). Note that low vimentin expression was observed following downregulation of HB-EGF unlike that following downregulation of WNT family genes. A scramble shRNA construct was used as the control.

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