Figure 6

Fluorescence of roGFP2 seedlings at different time points of a 1 mM AITC treatment. (A) Representative confocal images (BF: brightfield, GFP + PI: overlay image of GFP excited at 488 nm and propidium iodide fluorescent signals) of roots exposed to AITC for 24 h and 48 h. Intensive red staining in AITC-treated roots indicates cell death. Scale bars = 100 µm. (B) Representative pictures of WT and roGFP2 plantlets after 72 h of AITC treatment as under (A) demonstrate the AITC-triggered growth phenotype.