Figure 1 | Scientific Reports

Figure 1

From: Motility and chemotaxis of bacteria-driven microswimmers fabricated using antigen 43-mediated biotin display

Figure 1

Biotin display on the cell surface of E. coli via recombinant Ag43. (A) Schematic model of the Ag43-mediated peptide display. The N-terminus of Ag43 was modified with a FLAG epitope tag and a biotin acceptor peptide (BAP). ES: extracellular space, OM: outer membrane, PPS: periplasmic space. (B) E. coli cells carrying the recombinant Ag43-FLAG (induced with 100 µM isopropyl-β-D-thiogalactopyranoside (IPTG)) with and without BAP were analyzed using anti-FLAG immunostaining (red) and NeutrAvidin-biotin staining (yellow) for detecting recombinant Ag43-FLAG and surface-displayed biotin, respectively, and subsequent fluorescence microscopy. Scale bar: 4 µm.

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