Figure 2
Live imaging of CGNs asses the presence of neurotoxic PLA2 in V. aspis, but not in V. berus, venom. CGNs were treated with (A) β-BTx (0.25 µg/ml, 12,5 nM) or (B) V. aspis venom (35 µg/ml) or (C) V. berus venom (35 µg/ml) and pictures taken at indicated time points. V. aspis, but not V. berus, venom induces a bulging effect similar to β-BTx, hallmark of neurotoxic PLA2. Pictures show most significant frames coming from Supplementary Movie M1, M2 and M3 and are representative of a typical time course. Scale bar = 30 μm.
