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Figure 3

From: Nitazoxanide inhibits paramyxovirus replication by targeting the Fusion protein folding: role of glycoprotein-specific thiol oxidoreductase ERp57

Figure 3

Nitazoxanide causes F-protein insolubilization and aggregate formation. (A) IB for SeV-F, α-tubulin and histone-H3 in soluble and insoluble fractions of whole-cell extracts (WCEs) from mock-infected (Mock) or SeV-infected AGMK cells treated for 24 h with NTZ (5 μg/ml), 2.5 μg/ml tunicamycin (TM) or vehicle. Soluble and insoluble fractions from two separate experiments were processed as described in Methods. (B) IB for Flag-RSV-F, α-tubulin and histone-H3 in soluble and insoluble fractions of WCEs from HeLa cells transfected with RSV-F/ORF/C-Flag construct (RSV) or pcDNA3 empty vector (Mock), and treated for 20 h with NTZ (5 μg/ml), TM (2.5 μg/ml) or vehicle. (C) Confocal images of SeV-F (red) in mock-infected and SeV-infected A549 cells treated with NTZ (10 μg/ml) or vehicle for 24 h. Nuclei are stained with DAPI (blue). Merge and zoom images are shown. Scale bar, 7 μm (zoom, 3 μm). (A,B) Full-length blots/gels are presented in Supplementary Figs 12 and 13.

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