Figure 3
Overall effects of colistin exposure on colistin resistance Ecl8 cultures. (A) GO Terms derived from total differentially expressed genes in colR heteroresistant poplution of C2. C2 D3, D5 grown with colistin selection compared to D0 colistin naïve cultures to give colistin exposure and resistance, and D3, D5 grown with colistin compared to the same day grown without colistin selection to give colistin stress effects. The represented GO terms had a GSEA FDR-corrected p-value less than 0.01 in any comparison. Heatmap colors represent −log10 p-values obtained from GSEA analysis of differentially expressed genes. Red colors indicate GO terms with higher expression relative to the comparator, blue colors lower expression. (B) Scatter plot of expression changes compared to average expression of single the SNP crrB mutant grown with and (C) without colistin exposed. Each black dot represents a gene, and genes belonging to relevant enriched pathways are marked in the colour given by the key. The fold change cut-offs used are marked with a black line.
