Figure 5

Identify essential genes specifically involved in methanol supported cell growth. (A) A Venn diagram showing the overlap between putatively essential genes with NEP < 0.5 in methanol condition and putative non-essential genes in glucose condition (NEP > 0.5). (B) The growth rates (shown by spotting assay) and Aox enzymatic activities (shown by the colorimetrical assay) of the six mutants in glucose and methanol cultured conditions.