Figure 5 | Scientific Reports

Figure 5

From: Myoepithelial cell-driven acini contraction in response to oxytocin receptor stimulation is impaired in lacrimal glands of Sjögren’s syndrome animal models

Figure 5

Expression of Oxytocin receptor and smooth muscle proteins in isolated MECs. Lacrimal glands from SMA-GFP mice were digested and single cell isolates were used to sort GFP-expressing MECs. We used immunofluorescence (A,B) staining for SMA, calponin, and oxytocin receptor, of sorted MECs, to confirm expression and co-localization in GFP-expressing cells. (C) Proteins were isolated from sorted MECs and used for Western blotting to confirm the expression of SMA, calponin, and oxytocin receptor in lacrimal gland MECs. Cells stained with only the secondary antibody were used as a negative control (not shown). Scale bar represents 50 µm (B,C).

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