Figure 3
From: Dependence and Homeostasis of Membrane Impedance on Cell Morphology in Cultured Hippocampal Neurons
(a) Micrograph of a patterned neuron at 7 DIV during a patch-clamp recording. (b) Typical traces of the applied chirp current (top) and the voltage response (bottom). Only the first 10 s are shown. (c,d) Current-voltage relationships of (c) unpatterned (n = 6) and (d) patterned (n = 5) neurons at 7 DIV. The open circles and the solid line represent individual measurements and the mean, respectively. Magnification of the voltage range of −90 mV to −40 mV is shown in the right panels. (e) Effect of membrane potential (Vm) on the impedance magnitude profile (n = 6; 7 DIV; unpatterned). Error bars not shown to aid visualization. (f) Impedance spectra at various Vm obtained in the presence of TEA (n = 5; 7 DIV; unpatterned). (g,h) Impedance spectra of patterned (n = 5, red) and unpatterned (n = 5, black) neurons at (g) 7 DIV and (h) 16 DIV (Vm = −70 mV). Upper and lower panels show the impedance magnitude and phase profiles, respectively. Inset in (g) shows the impedance magnitude profile of patterned (n = 4) and unpatterned (n = 5) neurons recorded in the presence of TEA. Error bars indicate SEM. *p < 0.05; **p < 0.01 (one-sided t-test).
