Figure 1

DCs loaded tumor lysate (TL) were tested by Flowcytometer and RT-qPCR to check DCs capability to uptake tumor antigens and present them to Th1 cells. It showed that MHCII, CD40, and CD80 were significantly stimulated by tumor antigens (TL) in comparison to positive and negative controls (A,B). This is proved that DCs successfully uptake tumor antigens and proceeded presenting potency. Besides that, determination of specific time point to activate Th1 and CTL cells responses by tumor lysate was performed by Flowcytometer interval 3 hours; it showed a highest INF-γ⁺ accumulation at 24 hour, as showed in (C), CD4⁺INF-γ⁺ gated from CD3+ significantly raised after 9, 12, and 18 hours, but the highest response showed at 24 hours of incubation. While CD8⁺ INF-γ⁺ activation significantly rose at 18 hours and the highest response was noted at 24 hours of incubation (D), these results were confirmed by RT-qPCR to determine a relative INF-γ mRNA expression after activation of splenocytes by (TL = 20 ug/ml). Results showed that high response started at 18 hours to reach a highest at 24 hours (E). The statistical significant differences were represented from at least three independent experiments, ***P < 0.001.