Figure 2
An in vivo evaluation of 1-MT+TL/DCs-TL vaccination efficacy in C57BL/6 black mice challenged Pan02 tumor model showed that1-MT and 1-MT+TL/DCs-TL significantly reduced tumor size 2.144 cm3, 1.686 cm3, 2.166 cm3 respectively (A), tumor volume and weight also significantly reduced (C,D). Besides survival rate of 1-MT+TL/1-MT+DCs-TL treated groups was significantly improved (B), 38.64%, and 30.77% respectively in comparison with PBS group. Further Haematoxylin and Eosin (H&E) staining showed that, mice treated by 1-MT and 1-MT+TL (E.a,b) significantly represented many focal necrosis accompanied with more inflammatory infiltrating cells (black arrow for focal necrosis, and red arrow for inflammatory cells). Meanwhile 1-MT+DC-TL treated group (E.c) showed small tumor necrosis and few numbers of inflammatory infiltrating cells. Other tested groups (E.d,e,f and g) represented a small focal necrosis inside tumor tissue and a little number of inflammatory cells. The assessment of tumor fibrosis showed that 1-MT, 1-MT+TL, and 1-MT+DCs-TL groups extremely decreased collagen deposition folding rate in treated tumors as shown around pointed area (F.a,b,c), while other groups showed high collagen fibers (F.d,e,f,g). Besides that 1-MT treated groups showed significant immune reactivity of cleavage caspase3 pathway (G.a,b,c) that indicated promoting of tumor apoptosis. While other untreated groups showed no significant apoptotic reactions (G.d,e,f,g). These results indicated that administration of 1-Methyl-D-tryptophan and TAA vaccine destroyed tumor stromal and fibrosis, while it promoted significant apoptosis and cell death, which is very interesting observation. ***(P < 0.0001), and **(P < 0.001).
