Figure 9 | Scientific Reports

Figure 9

From: 1-Methyl-D-tryptophan Reduces Tumor CD133+ cells, Wnt/β-catenin and NF-κβp65 while Enhances Lymphocytes NF-κβ2, STAT3, and STAT4 Pathways in Murine Pancreatic Adenocarcinoma

Figure 9

Periphery of cellular and humoral immunological responses of all mice groups illustrated that, administration of 1-MT, 1-MT+TL, and 1-MT+DCs-TL significantly reduced Treg cells population (CD4+CD25+FOXP3+) in comparison to PBS group (4.76%, 1.12%, 8.7%, and 21.7%) respectively (A and C), while CD4+CD25− cells were significantly increased in comparison to PBS (73.9%, 74.7%, 74.2%, and 27.8%) respectively (A,B). ELISA analysis of periphery cytokines showed that circulating TGF-β was significantly reduced in the mice immunized by 1-MT, 1-MT+TL, and 1-MT+DCs-TL (D), while analysis of IL-12 showed significant rising in comparison to PBS (E). This is proved that tumor immunosuppressive pathways were completely blocked, while immunosurveillance and inflammatory immunomodulations were efficiently activated. Statistical significant differences were represented from at least three independent experiments, ***P < 0.001 and **(P < 0.001).

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