Table 2 Characterization of Tumor Markers Primers Used for RT-qPCR.
Gene | NCBI Gene ID | Orientation | Primer sequence (5′->3′) | Amplicon Size |
|---|---|---|---|---|
Ifng | 15978 | Forward | ATGAACGCTACACACTGCATC | 182 |
Reverse | CCATCCTTTTGCCAGTTCCTC | |||
Prom1 | 19126 | Forward | CTCCCATCAGTGGATAGAGAACT | 81 |
Reverse | ATACCCCCTTTTGACGAGGCT | |||
Cd274 | 60533 | Forward | GCTCCAAAGGACTTGTACGTG | 238 |
Reverse | TGATCTGAAGGGCAGCATTTC | |||
Gapdh | 14433 | Forward | AGGTCGGTGTGAACGGATTTG | 123 |
Reverse | TGTAGACCATGTAGTTGAGGTCA |
