Table 5 Primers of Signaling Pathways used for RT-qPCR.
Gene | NCBI Gene ID | Orientation | Primer sequence (5′->3′) | Amplicon Size |
|---|---|---|---|---|
Myd88 | 17874 | Forward | TCATGTTCTCCATACCCTTGGT | 175 |
Reverse | AAACTGCGAGTGGGGTCAG | |||
Traf1 | 22029 | Forward | AGGGTGGTGGAATTACAGCAA | 194 |
Reverse | GCAGTGTAGAAAGCTGGAGAG | |||
Traf6 | 22034 | Forward | AAAGCGAGAGATTCTTTCCCTG | 125 |
Reverse | ACTGGGGACAATTCACTAGAGC | |||
Irak4 | 266632 | Forward | CATACGCAACCTTAATGTGGGG | 125 |
Reverse | GGAACTGATTGTATCTGTCGTCG | |||
Jun | 16476 | Forward | TTCCTCCAGTCCGAGAGCG | 133 |
Reverse | TGAGAAGGTCCGAGTTCTTGG | |||
Ikbkb | 16150 | Forward | ACAGCCAGGAGATGGTACG | 297 |
Reverse | CAGGGTGACTGAGTCGAGAC | |||
Ikbkap | 230233 | Forward | CTGAAGTTGCATCGGACCCTG | 78 |
Reverse | CTCAGCTCGCAGACAGAAACA | |||
Stat3 | 20848 | Forward | CAATACCATTGACCTGCCGAT | 109 |
Reverse | GAGCGACTCAAACTGCCCT | |||
Stat4 | 20849 | Forward | TGGCAACAATTCTGCTTCAAAAC | 225 |
Reverse | GAGGTCCCTGGATAGGCATGT | |||
Nfkb1 | 18033 | Forward | ATGGCAGACGATGATCCCTAC | 111 |
Reverse | TGTTGACAGTGGTATTTCTGGTG | |||
Nfkb2 | 19697 | Forward | AGCGCGGGGACTATGACTT | 121 |
Reverse | GCCCGGTTATCAAAAATCGGAT | |||
Ctnnb1 | 12387 | Forward | ATGGAGCCGGACAGAAAAGC | 108 |
Reverse | CTTGCCACTCAGGGAAGGA |
