Figure 2 | Scientific Reports

Figure 2

From: Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation

Figure 2

Binding Analyses of anti-S. mutans and ant-S. sobrinus Fabs. (a) Fab ELISA of Fab SM-10, SM-12, SM-C-1, SM-C-4, SS-2, and SS-C-1 against S. mutans and S. sobrinus. E. coli stain DH10B and bovine serum albumin (BSA) were used as negative controls. Binding of Fabs to bacteria- and BSA-coated microtiter plates was detected using HRP-conjugated anti-His antibody. Error bars represent the standard deviation from three independent experiments. (b) Immunofluorescence of selected Fabs against S. mutans and S. sobrinus. Non-specific Fab against the human cell surface receptor EphB6 was used as a negative control. Binding of Fabs to bacteria-coated microtiter plates were detected via FITC-conjugated anti-FLAG antibody.

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