Table 1 Survey of different buffer conditions and under action of different proteins.
From: Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement
Solution | Drift | Best fit for \({\boldsymbol{\langle }}{{\boldsymbol{x}}}^{{\bf{2}}}{\boldsymbol{(}}{\boldsymbol{\Delta }}{\boldsymbol{t}}{\boldsymbol{)}}{\boldsymbol{\rangle }}\) |
|---|---|---|
Bare λ-DNA | No | \(0.21\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.04}\) |
λ-DNA + ATP | No | \(0.20\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.03}\) |
λ-DNA + ATP + Mg2+ + EDTA | No | \(0.22\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.94}\) |
λ-DNA + T4 DNA ligase | No | \(0.56\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.77}\) |
λ-DNA + T4 DNA ligase + ATP | No | \(0.18\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.96}\) |
λ-DNA + T4 DNA ligase + Mg2+ + EDTA | No | \(0.48\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.80}\) |
λ-DNA + T4 DNA ligase + ATP + Mg2+  + EDTA | Yes | \(0.23\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.77}+0.45\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.96}\) |
λ-DNA + T4 DNA ligase + ATP + Mg2+ + EDTA + BSA | No | \(0.38\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.92}\) |
λ-DNA + E-coli DNA ligase + NAD+ + Mg2+ + EDTA | Yes | \(0.17\mu {m}^{2}\,{({\rm{\Delta }}t\frac{1}{s})}^{0.46}+0.16\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.8}\) |
λ-DNA + E-coli DNA ligase + ATP + Mg 2 + + EDTA | No | \(0.07\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.19}+0.14\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.03}\) |
λ-DNA + MutS + Mg2+  + EDTA | No | \(0.11\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.79}\) |
λ-DNA + MutS + ATP + Mg2+ + EDTA | Yes | \(0.21\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.44}+0.49\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.95}\) |
λ-DNA + MutS + γ-ATP + Mg2+ + EDTA | Minimal | \(0.07\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{0.6223}+0.03\,\mu {m}^{2}{({\rm{\Delta }}t\frac{1}{s})}^{1.68}\) |
