Figure 3
(a) Characteristic 1D 1H NMR spectrum of the protein- and lipid-free intracellular metabolite extract of wild-type S. aureus recorded at 600 MHz (1H Larmor frequency) magnetic field strength with expanded regions of the spectrum displayed in panels (b–e). The NMR signals assigned to several metabolites are indicated. The insert (panel f) displaying the spectral patterns spanning the chemical shift range 2.6 to 2.85 ppm highlights how well the spectral features of aspartate can be fitted to its characteristic spectrum annotated in the ChenomxTM database (fit is in red) and compared to the experimental spectrum (shown in black). All NMR chemical shifts of the metabolite resonances are referenced to the DSS (sodium salt of 4,4-dimethyl-4-silapentane-1-sulfonic acid) signal set at 0 ppm.
