Figure 4

Effect of pregnancy on T cell populations in the spleen. Frequency of T helper cells (CD4⁺) (A), cytotoxic T cells (CD8⁺) (B), FoxP3⁺ Tregs (C), CD25⁺FoxP3⁻ effector CD4⁺ cells (D), CD69⁺ CD4⁺ cells (E), and CD69⁺ CD8⁺ cells (F) in the spleens of pregnant and non-pregnant BALB/c and B6 mice. T cytotoxic and T helper cells are expressed as the frequency of CD8⁺ and CD4⁺ cells within the CD3⁺ population respectively. Results are shown as dot plots + means and were tested for overall strain and pregnancy effects using a Two-way ANOVA, followed by a Bonferroni post-hoc test to test for strain specific pregnancy effects when interaction was found. Significant strain effects are indicated with solid lines and significant pregnancy effects are indicated with dashed lines (p < 0.05).