Figure 5
Cytosolic and secreted forms of Hsp70 do not enhance memory performance in young or elder flies. All genotypes were raised at 27 °C. Flies were trained at days 1 (a), 5 (b), 15 (c) or 30 (d) post-eclosion using olfactory classical conditioning and then tested immediately after training. Memory performance index (P.I.) is shown for control flies (ok107-Gal4/+; UAS-LacZ/+) and flies expressing either a secreted (ok107-Gal4/+; UAS-secHsp70/+) or a cytosolic (ok107-Gal4/+; UAS-cytHsp70/+) form of the Hsp70 chaperone. Memory performance of flies expressing the secreted (ok107-Gal4/+; UAS-secHsp70/+; day 1, p = 0.0550; day 5, p = 0.2960; day 15, p = 0.4265; day 30, p = 0.5554) or the cytosolic (ok107-Gal4/+; UAS-cytHsp70/+; day 1, p = 0.1339; day 5, p = 0.7947; day 15, p = 0.6182; day 30, p = 0.8622) form of Hsp70 is statistically undistinguishable from the performance of control flies (ok107-Gal4/+; UAS-LacZ/+) at all ages tested. Error bars indicate SEM; n = 14 per group; Tukey’s comparison test: no significant.
