Figure 1
From: Adolescent alcohol exposure epigenetically regulates CREB signaling in the adult amygdala
Effects of adolescent intermittent ethanol (AIE) exposure on CREB and pCREB protein levels and Creb1 mRNA levels in the amygdala of adult rats. (a) Representative photomicrographs (Scale bar = 50 μm) of CREB and pCREB gold immunolabeling in the amygdaloid brain structures of AIE and adolescent intermittent saline (AIS) exposed adult rats, and (b) bar diagram showing quantification of CREB and phosphorylated CREB (pCREB) gold immunolabeling in the central (CeA), medial (MeA) and basolateral amygdala (BLA) of AIE and AIS adult rats. Values are presented as the mean ± SEM of the number of immuno-gold particles/100 μm2. (c) Bar diagram showing fold changes in mRNA levels of Creb1 in the amygdala of AIS and AIE adult rats. Values are presented as the mean±SEM of the fold changes derived from AIS control rats. **p < 0.01, ***p < 0.001, Student’s unpaired two-tailed t-test, n = 6/group (Gold immunolabeling), n = 6/group (mRNA studies).
