Figure 1 | Scientific Reports

Figure 1

From: Cellular dissection of malaria parasite invasion of human erythrocytes using viable Plasmodium knowlesi merozoites

Figure 1The alternative text for this image may have been generated using AI.

Double filtration of A1-H.1 P. knowlesi E64-arrested schizonts isolates merozoites of high purity. (a) Representative flow cytometry plots showing different cell populations, pre and post filtration. Plots do not show uninfected RBCs (y-axis has been cropped to give better resolution of free merozoite populations). Figure S3 shows uncropped RBC population. Free merozoite population was tightly-gated after identification of haemozoin-derived events (Fig. S1). (b) Double-filtration increases the merozoite:late-stage ratio significantly, giving a free merozoite preparation of high purity. Graph shows mean from n = 9 experiments. Error bars represent SEM. P-values comparing different merozoite:late-stage ratios represent one-way multiple comparisons ANOVA test (**P < 0.01). (c) TEM image of free merozoites. Scale bars = 500 nm.

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