Figure 4
Effect of Mg conditioned media on hMSCs metabolic activity and DNA concentration was investigated using AlamarBlue assay and PicoGreen assay over a period of 7 days. hMSCs were cultured in the presence of various concentrations of Mg conditioned medium, (A) filtered medium and (B) non-filtered medium. A reduction in metabolic activity was observed when cells were cultured in Mg100 non-filtered medium on day 3 and 7 in comparison to the control. DNA concentration of hMSCs was also investigated following culture in (C) filtered medium and (D) non-filtered medium. A significant decrease (#p < 0.001) in DNA concentration was observed when cells were cultured in Mg100 non-filtered medium on day 7. However, when the Mg non-filtered medium concentration was reduced to Mg25 and Mg10 a significant increase (#p < 0.001) in DNA concentration was observed. The data is represented relative to the control (100%). The dotted line represents the cytotoxicity threshold on day 7. The bars represent the mean and standard deviation in the positive orientation of three independent experiments, each with n = 3. Representative images taken using Cell IQ live imaging system shows cells cultured in non-filtered medium (Mg50) at (E) 0 hrs (F) 24 hrs and (G) 48 hrs and (Mg100) at (H) 0 hrs (I) 24 hrs and (J) 48 hrs. (K) Image showing cells cultured in standard growth medium (control) at 48 hrs. Scale bar = 100 µm, arrows indicate the presence of cell aggregates.
