Figure 7
The effect of Mg conditioned medium on RAW cell metabolic activity was investigated using AlamarBlue assay. RAW cells were cultured in the presence of various concentrations of Mg conditioned medium, (A) filtered medium and (B) non-filtered medium. A reduction in metabolic activity was observed when cells were cultured in both Mg100 filtered and non-filtered medium over the 3 day culture period compared to the control, however culture in Mg100 non-filtered medium had the greatest effect. Culture in Mg50 filtered and non-filtered medium also resulted in a reduction in metabolic activity at day 1 and 2 compared to the control. The data is presented relative to the control (100%). The bars represent the mean and standard deviation in the positive orientation of three independent experiments, each with n = 3.
