Figure 8
Effect of Mg conditioned media on osteoclast related genes. RAW cells were cultured in the presence of Mg conditioned medium at varying concentration with (non-filtered medium) or without (filtered medium) the presence of corrosion granules for a period of 5 days. Fold change in the expression of (A) TRAP and (B) NFATC-1 after treatment with Mg conditioned medium was investigated. No significant difference in gene expression was detected between the treated conditions and positive control. (C) TRAP assay was performed following culture in Mg conditioned medium in the presence of RANK-L. All TRAP positive multinucleated cells in each well were counted for analysis. Culture in Mg50 filtered and non-filtered medium resulted in a significant reduction in cell number (**p < 0.01) compared to the positive control. A significant difference (*p < 0.05) in cell number was also observed in cells cultured in Mg50 non-filtered medium compared to Mg25 non-filtered medium. The bars represent the mean and standard deviation in the positive orientation of three independent experiments, each with n = 8. The fold change is relative to the untreated sample (without RANK-L or Mg). Positive control represents RAW cells cultured in normal growth medium and then treated with RANK-L to induce differentiation. (D) Representative images of TRAP positive multinucleated cells taken after 5 days of culture are presented. The arrow represents undifferentiated RAW cells. Images from three independent experiments were analysed, each with n = 8.
