Figure 9
Effect of Mg non-filtered medium on mature osteoclast activity. (A) The number of TRAP positive mature osteoclast cells present on bone slices following a 24 hr treatment with various concentration of Mg non-filtered conditioned medium is presented. Mature osteoclast cell number was normalised relative to the standard control (100%). (B) Resorption pits formed by mature osteoclasts in the presence of Mg non-filtered medium were counted and normalised to osteoclast cell number. Resorption activity was normalised relative to the standard control (100%). Cells cultured in MO standard medium with the presence of sCT (inhibitor of resorption activity) were used as the positive control. The bars represent the mean and standard deviation in the positive orientation of three independent experiments, each with n = 6.
