Figure 1
From: Annexins induce curvature on free-edge membranes displaying distinct morphologies
Typical configuration of a membrane stack prepared by hydration of a spincoated lipid film as used in the present study (top). The response of an open edged membrane patch is monitored by time-lapse fluorescence imaging after exposure to annexins. Sequence (a–d) and sequence (i–l) show the response of an open edged membrane (DiDC18) to exposure to ANXA1 and ANXA2 respectively. Contraction and blebbing from the patch is evident in both cases and folding is also evident for ANXA1. Sequence (e–h) shows the GFP channel (sfGFP-ANXA1) demonstrating binding of ANXA1 to the membrane. Red arrows indicate initial defect sites, the green arrow is a fold structure and the yellow arrows are blebs. Response of a membrane patch to ANXA6 (m–p). The membrane responds by modifying the free membrane edges and by creating folds as characterized by bright elongated structures. Nucleation of membrane holes inside the membrane patch is observed in parallel with folding. Concentrations: ANXA1-sfGFP: 46 nM, ANXA2-sfGFP: 58 nM, ANXA6: 50 nM.
