Figure 2

Response of membrane patches to exposure to ANXA4 (a–d) and ANXA5 (e–h) respectively. In both cases a complete roll-up of the membrane patch is observed as initiated from points on the free edges. Analysis of the rolling dynamics was performed by quantification of the incremental area reduction between image frames as shown graphically in (i). Alternating bright and dark grey bands (i) show the area reduction during rolling. The rolling dynamics plotted as the cumulative rolled area (j) and the time constant τ derived from fit to a logistic function (k). Histograms in (k) are based on n = 5 repeats. A logistic function was fitted to the rolled area (j) which provides a characteristic time scale τ for the rolling dynamics. Fluorescence image of a roll pattern induced by ANXA4 and the corresponding AFM topography image of the same region (l,m). AFM image of the primary membrane after exposure to ANXA4 showing patterns of bound ANXA4 (n). Line profiles of the AFM images (o). Concentrations: ANXA4: 43 nM, ANXA5: 44 nM.