Figure 5
From: Annexins induce curvature on free-edge membranes displaying distinct morphologies
Response of membrane patches to ANXA7 and ANXA11 which both induce very similar types of membrane modifications. Imaging of the DiD membrane probe shows reduction of the patch area and formation of characteristic bright lens structures for ANXA11-sfGFP (a–d) and for ANXA11 (j–m). Imaging in the GFP channel of ANXA11-sfGFP shows that ANXA11 is being incorporated into the lens structures (e–h) and depleted from the membrane surface (i). TopFluorPS labeling shows that the anionic PS lipid localizes to the lens structures (n–q). Comparison with results for ANXA7 (r–u) displaying both reduction in area and lens formation, equivalent to the observations for ANXA11. Concentrations: ANXA11-sfGFP: 31 nM, ANXA11: 32 nM, ANXA7: 38 nM.
