Figure 6

Fluorescence (DiD) image of lens structures formed in a membrane patch after exposure to ANXA7 (a). The square indicated in (A) was subsequently scanned with AFM which provides the topography of the lens structure (b). Selected line profiles in the AFM topography show the bilayer step height (c) and the height variation across a lens (d). Conceptual model for lens formation (e–g). Model for the formation of hydrophobic reverse micelles containing ANXA7 + POPS that may constitute the interior of a membrane lens (g). Fluorescence sequence (DiD) illustrating the fusion of two ANXA7-induced lenses (h–j) and the subsequent rupture of the combined lens (k–m). Concentrations: ANXA7: 38 nM.