Figure 3

Increase in circulatory inflammation and alteration of gene expression in CD163⁺macrophages of non-survivors in SAH. (A) Circulatory iron in Non-survivors (n = 56;856.9[109.4-2744]ug/mL) vs. Survivors (n = 44; 312.5[109.4-1134] ug/mL) [p ≤ 0.01]. TNF-α levels in Non-survivors (80(28-118) pg/mL) vs. Survivors (39.5(28-86) pg/mL) [p ≤ 0.01]. (B) sCD163 levels in Non-survivors(1583[595-2216]ng/ml) vs. Survivors(590[180-1235]ng/ml) [p ≤ 0.01].He-Hp complex levels in Non-survivors(2[1.4–20]ng/ml) vs. Survivors(4.5[1.5–23]ng/ml) [p ≤ 0.05]. (C) Intracellular peroxide levels of CD163⁺ cells in (n = 10) Non-survivors 2750[2500–3300] MFI vs. (n = 10) Survivors 2250[2000–2550] MFI (p < 0.01). (D) Relative expression of oxidative-stress, inflammation and iron-regulatory genes in CD163⁺ cells of Non-survivors (n = 3) vs. Survivors (n = 3) ([*] = p < 0.05). (E) Relative expression of ADAM17, CD163 and linked genes in CD163⁺ cells of Non-survivors vs. Survivors ([*] = p < 0.05). (F) Relative expression of apoptosis, ER-stress and autophagy genes in CD163⁺ cells of Non-survivors vs. Survivors ([*] = p < 0.05).