Figure 7
From: The small molecule drug diminazene aceturate inhibits liver injury and biliary fibrosis in mice
Diminazene aceturate (DIZE) inhibited reactive oxygen species (ROS) production and lipid peroxidation. Increased ROS production by TGF-β1 activated human hepatic stellate cells, LX-2 cells, was inhibited by incubation with DIZE. (A) ROS production by LX-2 cells was measured in a multiplate reader using 2′,7′-dichlorodihydrofluorescein diacetate. Increased oxidation of cell membrane lipids by ROS in the diseased liver was abrogated by DIZE, as measured by immunohistochemical localization of 4-hydroxynonenal (4-HNE) adducts. (B,C) Each bar represents the mean ± SEM profile from 3 experiments (A) or 10–15 mice per treatment group. (B) Representative images from the 3 groups are shown in panel C.
