Figure 4

Nonfunctional il10 does not increase the expression of proinflammatory cytokines at the early stages of a low-dose (2–9 CFU) mycobacterial infection in zebrafish. (a–d) The relative expression levels of il10 and proinflammatory cytokine genes (il1b, tnfa and tnfb) were measured in the abdominal organ blocks (including kidney) of il10e46/e46 mutant fish (n = 6–12) and WT controls (n = 5–12) at 1 dpi and 6 dpi and are presented as a scatter dot plot and median. Note the different scales of the y axes. Gene expressions were normalized to the expression of eef1a1l1. The data were collected from a single experiment. A two-tailed Mann-Whitney test was used for the statistical comparison of differences.