Figure 5
Second Harmonic Microscopy images of BiFeO3 nanoparticle aggregates immobilized in agarose (top row), with the corresponding two-photon emission spectra (bottom row): (a) using mucic acid without NaCl or glycerol but where combustion was prevented; (b) using mucic acid with NaCl and glycerol and with the unavoidable combustion step; and (c) using mucic acid and NaCl where combustion was prevented. Each image is a composite of 25 colours (from violet to red, corresponding to idenpendent detection channels): excitation was at 810 nm. Violet represents second harmonic (405 nm), white corresponds to the mix of other colours and represents two-photon excited luminescence. For sample b, the two-photon excited fluorescence (TPEF) signal acquired in the squared Region of interest (ROI) is plotted below in red in the corresponding spectrum.
