Figure 1 | Scientific Reports

Figure 1

From: Improving catalytic activity of the Baeyer–Villiger monooxygenase-based Escherichia coli biocatalysts for the overproduction of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid

Figure 1

Time course of the biotransformation of ricinoleic acid by the recombinant E. coli BL21(DE3) pAPTm-E6BVMO-ADH, expressing an alcohol dehydrogenase (ADH) of Micrococcus luteus and the engineered Baeyer-Villiger monooxygenase (BVMO) from Pseudomonas putida KT2440 (i.e., E6BVMO). The biotransformation was initiated by adding 15 mM ricinoleic acid and 0.5 g/L Tween80 at t = 0 into the recombinant E. coli culture broth (cell density: 3 g cell dry weight (CDW)/L). Ricinoleic acid was added once more to a concentration of 15 mM at t = 5 h. Symbols indicate the concentrations of ricinoleic acid (1) (●), 12-keto-octadec-9-enoic acid (2) (△), and the ester (3) (■). The experiments were performed in triplicate. The error bars indicate standard deviations.

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