Figure 5 | Scientific Reports

Figure 5

From: Improving catalytic activity of the Baeyer–Villiger monooxygenase-based Escherichia coli biocatalysts for the overproduction of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid

Figure 5

Time course of the biotransformation of ricinoleic acid by the recombinant E. coli BL21(DE3) pAPTm-E6BVMOC302L-ADH, expressing the ADH of M. luteus and the engineered E6BVMO (i.e., E6BVMOC302L) (A) and the in vitro activity of E6BVMOC302L, which was prepared from the cells at t = 0, 4, and 6 h during the biotransformation shown in (A,B). The whole-cell biotransformation was conducted at the same condition as the biotransformation shown in Fig. 1. The in vitro activity of E6BVMOC302L at t = 0 was 0.050 U/mg proteins. Symbols indicate the concentrations of ricinoleic acid (1) (●), 12-keto-octadec-9-enoic acid (2) (△), and the ester (3) (■). The experiments were performed in triplicate. The error bars indicate standard deviations.

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