Figure 6
Time course of the biotransformation of ricinoleic acid by the recombinant E. coli BL21(DE3) pAPTm- E6BVMOC302L-ADH, pACYC-FadL expressing the long chain fatty acid transporter FadL in the outer membrane in addition to the ADH and E6BVMOC302L. The biotransformation was initiated by adding 150 mM ricinoleic acid and 0.5 g/L Tween80 into the recombinant E. coli culture broth (cell density: 25 g CDW/L). Glucose was added into the cultivation medium to a rate of 5 g/L/h to facilitate regeneration of the nicotinamide cofactors in E. coli cells. Symbols indicate the concentrations of ricinoleic acid (1) (●), 12-keto-octadec-9-enoic acid (2) (△), and the ester (3) (■). The experiments were performed in triplicate. The error bars indicate standard deviations.
