Figure 4

Induction of expression and activation of p53 in C2C12 cells by Mct1 knockdown. (a–c) Control and Mct1 siRNA-introduced C2C12 cells were cultured for the indicated periods in the absence (−) or presence (+) of BMP-2 (300 ng/mL). Expressions of mRNAs for Klf4 (a), Trp53 (b), and p21 (c) were analyzed by real-time RT-PCR, then normalized against that of Gapdh, with the results indicated as relative values. (a–c) Values are expressed as the mean ± SD (n = 3–5). (d) C2C12 cells were introduced with control or Mct1 siRNA, then p53 protein was detected using an immunocytochemical method. Nuclei were stained with DAPI. (e) Ratios of p53-positive nuclei in cultures of C2C12 cells introduced with control or Mct1 siRNA. (a–c,e) Data are expressed as the mean ± SD (n = 3–5). **Significantly different from control group (*p < 0.05, **p < 0.01).