Figure 1
From: Inhibition of the precursor and mature forms of HIV-1 protease as a tool for drug evaluation
(A) Schematic representation of the uncleaved mCherry-PRprec-EGFP fusion protein: Two fluorescent proteins, mCherry and EGFP, are linked by an artificial HIV-1 PR precursor containing HIV-1 transframe peptide (TFP), p6* peptide, an HIV-1 PR monomer and a N-terminal fragment of reverse transcriptase. HIV-1 PR cleavage sites are marked with asterisks. (B) LEFT: Western blot analysis of HEK293T cell lysates detected with anti-mCherry antibody (green) (the arrow denotes the uncleaved precursor): Lanes: 1. Marker 2. Untransfected cells 3. Cells transfected with reporter harboring wild type PR without inhibitor. 4. Cells transfected with reporter harboring PR treated with 80 nM darunavir. 5. Cells transfected with reporter harboring wild type PR treated with 10 μM darunavir. RIGHT: Western blot analysis of cell lysates detected with anti-GFP antibody (green) – Lanes are the same as in the left panel. Detection of β-actin was used as a loading control (red).
