Figure 2
From: Inhibition of the precursor and mature forms of HIV-1 protease as a tool for drug evaluation
Fluorescent life-time imaging (FLIM) indicates FRET between EGFP and mCherry in the mCherry-PRprec-GFP precursor. (A) Schematic representation of the autoprocessing inhibition assay based on expression of the mCherry-PRprec-GFP reporter. (B) Quantification of the FRET level based on life-time comparison of 1 μM darunavir (DRV)-treated and untreated cells (C). Fluorescent images of HEK293T cells transfected with the mCherry-PRprec-EGFP reporter inhibited/non-inhibited with 1μM DRV: green and red channels and a fluorescent life-time image. The scale bar indicates 5 µm. The error bar represents the standard error of the mean. The FLIM experiment was performed for 10 cells in both conditions.
