Figure 5
From: Inhibition of the precursor and mature forms of HIV-1 protease as a tool for drug evaluation
Screening of HIV-1 protease inhibitors: HEK293T cells were transiently transfected with the mCherry-PRprec-EGFP reporter and treated with 400 nM of each inhibitor in triplicate. Darunavir was used as a reference compound. After 24 h, the cells were harvested and analyzed by flow cytometry. The mCherry signal corresponds to the total inhibition of all PR forms (precursor, partially cleaved precursor and mature form), whereas the FRET signal corresponds to the amount of PR precursor. The mean fluorescence (F) for the appropriate inhibitor was divided by the fluorescence (F) obtained for darunavir. The error was expressed as a standard error.
