Figure 4

Distinct clusters of correlated, co-expressed genes emerge from network analysis of transcriptomes from stress-stratified microglia. (a) A transcript-to-transcript correlation network graph of transcripts significantly differentially expressed (DE) by social defeat was generated in Miru (Pearson correlation threshold r ≥ 0.80). Nodes represent gene transcripts, and edges represent the degree of correlation in co-expression. The network graph was clustered using a Markov clustering algorithm, and transcripts were assigned a color according to cluster membership. This resulted in 14 clusters containing >10 nodes. (b) Mean unit variation (MUV) profile of all transcripts that compose the four largest clusters. (c) Heat maps showing the expression profile of all transcripts contained within these clusters. Z-scores of individual probe sets are represented by color with red indicating high expression and blue indicating low expression.