Figure 7
Microglia from CSD-S mice have expression profile seen in inflammation, neurodegenerative disease, aging, and response to albumin. Microarray data were analyzed using gene set enrichment analysis (GSEA) software to identify functionally related gene sets with statistically significant enrichment in stressed microglia compared with several neuroinflammatory models. (a) Plots show GSEA enrichment in array data from CSD-S microglia compared with Cortex response to albumin infusion93, BBB response to cytokines75, and elevation in aging44 as the expression phenotype. The plots show the distribution of genes in each set that are positively and negatively correlated with the respective responses. (b) Gene sets from CSD-S but not CSD-R microglia highly correlate with gene sets from several models of neuroinflammation75,93,94,95,96, neurodegeneration40,41,42, and aging44,74.(c,d) Heat maps detailing the microarray expression patterns of unique LPS-induced (c) and IL-4 induced (d) genes46 that were differentially expressed across HC, CSD-S, and CSD-R microglia. Row- z-score intensity was used to represent expression differences across groups. NES, normalized enrichment score; H, HC; R, CSD-R; S, CSD-S. p-value, nominal p value.
