Figure 1

(a) Representative Immunofluorescent staining of DAPI, Pax7, CD56 (a, top), CD29 (a, bottom) on an adult bovine muscle fibre. Scale bars: 25 μm. (b) FACS analysis of mononuclear cells from biceps femoris obtained from adult cow. Cells were gated by forward scatter and side scatter (not shown) prior to gating for Hoechst, CD45/31, CD56/29. Red gates (ovals) indicate sub-populations containing bovine satellite cells. (c) qRT-PCR analysis of PAX7 mRNA levels in total cells (not sorted), sorted satellite cells and satellite cells depleted populations after a period of 4 days in culture. (n = 3). (d) Representative Immunofluorescent staining of PAX7 in sorted bovine satellite cells cultured for 4 days. Scale bars: 25 μm. (e) Quantification of PAX7 immunofluorescent staining of sorted CD56⁺CD29⁺ cells and unsorted cells cultured for 4 days (n = 3). Data are represented as mean ± SEM. Significance was analyzed by Student’s t-test for 2 groups, One-way ANOVA with Bonferroni’s Multiple Comparison Test for more than 2 groups. Asterisks: ***indicates P < 0.001.